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Ncy exists or not, that the Cul5-Roc2 complex has been evolutionarily conserved suggests that it plays an important role in many organisms.Materials and Methods Cell Culture and TransfectionS2 cells were maintained in Schneider's medium supplemented with 10 FBS and 100 U/ml penicillin and 100 ug/ml streptomycin. Cells were transfected using Effectene according to the manufacturer's instructions (
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He only successful therapeutic strategy; however, patients with unresectable or metastatic disease survived only a median of 18-24 months after diagnosis [4,5]. Those patients with widespread metastatic disease have an estimated 9 month overall survival [6]. The development of the selective kinase inhibitor imatinib mesylate (also known as Gleevec) has dramatically altered the treatment strategies
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]. Interestingly, the BNAR chimera, which binds well to Cul3, effectively rescues Roc1b mutant male sterility. This is consistent with our previous data showing that normal Roc1a can partially rescue the male sterility of Roc1b mutants when expressed from the Roc1b promoter [25]. These data suggest that in the context of the testes specific Cul3 complex, the RING domains of Roc1a and Roc1b can pro